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Harp PM, Hahn BH: Origins of HIV and the AIDS Pandemic. Cold Spring Harb Perspect Med 2011, 1:a006841. 3. Brennan CA, Bodelle P, Coffey R, Devare SG, Golden A, Harris B, Holzmayer V, Luk KC, Schochetman G, Swanson P, Yamaguchi J, Vallari A, Ndembi N, Ngansop C, Makamche F, Mbanya D, Gurtler LG, Zekeng L, Kaptue L, Hackett J Jr: The prevalence of diverse HIV-1 strains was stable in Cameroonian bloo
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Equences previously identified as belonging to these known clades by constructing maximum likelihood trees from all available gag and nef sequences for each clade, and selecting one sequence from each of the up to ten most basal lineages from the root of these clades. Anonymously-donated HIV-infected blood units were collected between December 2006 and August 2007 from Yaound?Central Hospital, Cam
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Bited a 325-bp PCR product, whereas SR-AI/II-/- mutant allele showed a 434-bp PCR product. MARCO wild type allele exhibited ca. 500-bp PCR product, and MARCO-/- mutant exhibited ca. 850-bp PCR product. All of the three cell lines are stable and Mycoplasma-free by Mycoplasma PCR ELISA test (Roche, Indianapolis, IN) during culture in the past 24 months.SR-AI/IIM WT ZK1 ZK2 ZK6 MMARCOWT ZK1 ZK2 ZKMAR
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Pacity of latex beads similar to primary AMs deficient in MARCO and SR-AI/II. All three clones showed significantly decreased uptake of fluorescent latex beads compared to the wild type primary AMs (Fig. 6A). Observed differences in phagocytic capacity between these clones and parental primary AMs from MS-/- mice may reflect the heterogeneity seen in populations of primary alveolar macrophages.ZK1
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Orrespondence: Darrin.Martin@uct.ac.za; Wendy.Burgers@uct.ac.za 3 Computational Biology Group, Institute of Infectious Diseases and Molecular Medicine, University of Cape Town, Cape Town, South Africa 1 Division of Medical Virology, University of Cape Town, Cape Town, South Africain west central Africa, at 5.3 [8]. This, together with the co-circulation of divergent variants of multiple clades, h
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R macrophages which was derived from brochoalveolar lavage (BAL) obtained from MS-/- mice [31]. Immortalization was conducted by infection of the primary AMs from MS/- mice with a retrovirus J2. The immortalized AMs were cloned by limiting dilution method. Three of the clones, designated as ZK-1, ZK-2 and ZK-6 were chosen for further characterization of macrophage phenotype and phagocytic function
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Bited a 325-bp PCR product, whereas SR-AI/II-/- mutant allele showed a 434-bp PCR product. MARCO wild type allele exhibited ca. 500-bp PCR product, and MARCO-/- mutant exhibited ca. 850-bp PCR product. All of the three cell lines are stable and Mycoplasma-free by Mycoplasma PCR ELISA test (Roche, Indianapolis, IN) during culture in the past 24 months.SR-AI/IIM WT ZK1 ZK2 ZK6 MMARCOWT ZK1 ZK2 ZKMAR
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Bited a 325-bp PCR product, whereas SR-AI/II-/- mutant allele showed a 434-bp PCR product. MARCO wild type allele exhibited ca. 500-bp PCR product, and MARCO-/- mutant exhibited ca. 850-bp PCR product. All of the three cell lines are stable and Mycoplasma-free by Mycoplasma PCR ELISA test (Roche, Indianapolis, IN) during culture in the past 24 months.SR-AI/IIM WT ZK1 ZK2 ZK6 MMARCOWT ZK1 ZK2 ZKMAR